Hi,
There are a couple of options that could help with this but I think the best approach would be to create a new field as a calculated field. This new calculated field would be defined as a string parse using the TP calc function LEFT,1. The source field for this new calc field should be defined as your barcode field. From this calc field, you can do the validation to match "E" and on a failed validation, you can even direct the field to set focus back to your barcode field. Of course, this new calc field does not have to be visible or even part of the form but can still be used to validate as you need.
Another option is worth mentioning just in case you weren't aware of it. This 2nd option involves our Conditional Logic area which can be configured via the Form Designer toolbar (the last button on the right hand side of the toolbar. Conditional logic allows for alot of custom actions to take on definable events along with definable actions to take; like Alert messages, etc.
If nothing else, option 1 is probably your best bet but check out conditional logic as well; there is a lot of power in that area of TracerPlus.
Please note that Conditional logic is available for TP v9 and later.
Thanks.